Blood specimens

Test name Type of Blood Sample Special instructions
Malaria species identification,
TAT = 1 day
Unclotted, EDTA blood Clotted blood is unsuitable
Parasite count/parasitaemia,
TAT = 1 day
Unclotted, EDTA blood Clotted blood is unsuitable
Antigen test for Plasmodium falciparum,
TAT = ½ day
Unclotted, EDTA blood Clotted blood is unsuitable
Antibody test for malaria Not performed in parasitology
Microfilaria or Filaria or Wuchereria bancrofti or elephantiasis or Loa loa,
TAT = 1 day
Unclotted, EDTA blood Blood sampling should be performed at the correct times depending on the suspected filarial species.
Antigen test for Wuchereria bancrofti (lymphatic filariasis or elephantiasis), TAT = ½ day Unclotted, EDTA blood Clotted blood is unsuitable
Sleeping sickness or Trypanosomes,
TAT = 1 day
Unclotted, EDTA blood, Fresh CSF Clotted blood is unsuitable
Relapsing fever (Borrelia duttoni or B. recurrentis),
TAT = 1 day
Unclotted, EDTA blood Clotted blood is unsuitable
Babesia,
TAT = 1 day
Unclotted, EDTA blood Clotted blood is unsuitable

 

Stool specimens

Test name Special instructions
Identification of all human nematodes including:

Ascaris lumbricoides (common roundworm)
Hookworms (Ancylostoma duodenale/ Necator americanis)
Trichuris trichiura (whipworm)
Strongyloides stercoralis

None

TAT = 1 day

Specimens, that may take longer than a day to reach the laboratory, should ideally be preserved in an equal quantity of 10% formalin

Identification of all human cestodes including:

Tapeworm (Taenia saginata/solium)

TAT = 2 days
Identification of all human trematodes including:

Bilharzia (Schistosoma mansoni/haematobium)

TAT = 1 day
Identification of all human protozoa including:

Isospora belli/ hominis
Cryptosporidium parvum
Cyclospora cayetanensis
Giardia lamblia
Blastocystis hominis

TAT = 1 day
Microsporidia species identification TAT = 3 days
Amoebae (including Entamoeba histolytica) Specimen must reach laboratory within 30 minutes of sampling. Don’t add preservative.
TAT = 1 day for microscopy

 

Other types of specimens

Type of specimen Test Special instructions
Induced sputum or bronchial washings or BAL (bronchoalveolar lavage)
TAT = 1 day
IFA for PCP (Pneumocystis jiroveci, previously known as carinii) Sputum is not an optimal specimen. BAL is preferable.
Sputum
TAT = 1 day
Larvae of Strongyloides stercoralis Larvae may be infective: handle with care
Urine
TAT = 1 day
Bilharzia (Schistosoma haematobium); Trichomonas vaginalis At least 5 ml urine is required
Sticky tape swab
TAT = 1 day
Pinworm (Enterobius vermicularis) Early morning sticky tape swab is the optimal specimen. Eggs may be infective: handle with care
Liver abscess fluid or cyst fluid
TAT = 1 day
Amoebic liver abscess (Entamoeba histolytica) Specimen must reach laboratory within 30 minutes of sampling
Cyst fluid/ sputum
TAT = 1 day
Hydatid hooklets and scolices of Echinococcus species None
Worm or proglottid (egg packet)
TAT = 1 day for worm ID, 2 days for proglottids
Worm and tapeworm identification Submit proglottids in saline
Bone marrow or liver biopsy or skin biopsy/ impression smears
TAT = 2 days
Leishmania species Submit skin biopsy in saline
Corneal scrapings or biopsy or contact lenses & solutions
TAT = 1 month
Acanthamoeba keratitis Contact Parasitology Unit before taking samples to make arrangements for sampling & culture